Reporter
Part:BBa_K3190205:Design
Designed by: Ojas Tulsyan Group: iGEM19_UCopenhagen (2019-10-14)
sfGFP: Superfolder GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 13
Design Notes
We use the coding sequence of sfGFP to add it to the C-terminus of the coding sequence of the receptor along with a linker. This is done to carry out a co-localisation assay, to determine the protein expression and to confirm the alignment of the receptor molecule.
Source
Aequorea victoria